IVF Center In Chakan

The newest of the aspiration techniques is testicular sperm retrieval (TESA – TESE). In this procedure, a small amount of testis tissue is taken by biopsy under local anesthesia. It is a breakthrough in that it demonstrates that sperm do not have to “mature” and pass through the epididymis in order to fertilize an egg. Because of their immaturity, however, testicular sperm need ICSI.

Testicular sperm extraction is indicated for patients in whom there is a blockage in the epididymis very near the testis (either from prior surgery, infection or from birth), or a blockage within the ducts of the testes (efferent ductules).

It is also used for men with extremely poor sperm production, in which so few sperm are produced that they cannot reach the ejaculate. Pregnancies are now routine in cases of poor sperm production, but there is some concern with the use of this sperm because in most cases the underlying condition causing the poor sperm production is still unknown.

Therefore, in these cases, it must be realized that the condition which may have caused the infertility, may be transmitted to the progeny. Recently, even spermatids (round cells that eventually become a sperm with a tail) have been used to achieve pregnancies with ICSI.

However, this has raised much speculation and concern about the use of genetic material from a still-evolving germ cell for clinical purposes before the system has been appropriately investigated and its genetic stability examined in animal models.

Spermatid injections are currently considered experimental procedures. One drawback of testis sperm is that is does not freeze as readily as epididymal or vasal sperm and thus it is more likely that the male partner will need to undergo repeated procedures for each IVF attempt.

When is TESA and PESA carried out?

TESA and PESA are usually carried out when a man’s ability to naturally release sperm via ejaculation is reduced by blockage of the vas deferens or epididymis. Though, PESA involves collecting sperm via a small needle that is inserted into the epididymis while TESA involves collecting sperm via a small needle that is injected directly into the testicles. Some of the conditions that may require TESA and PESA to be performed are discussed below.


If after all the PESA procedures have been performed and no sperm is present in the vas deferens or epididymis, then TESA is performed. A small needle will be injected directly through the testes, into the testicles and testicular tissue aspirated by having the plunger drawn out. Just like in PESA, sufficient quality and quantity of sperm should also be retrieved using TESA. PESA and TESA are a great option for couples, much better than Intrauterine Insemination using donor sperm as it also gives the man the golden chance to contribute to their offspring’s genetic structure.

Benefits of TESA and PESA

IMost men who suffer from infertility usually assume that they are completely sterile and they can never impregnate a woman. What most of them forget is that there could be a condition preventing sperms from getting out of their reproductive system during ejaculation. PESA and TESA will determine exactly where the issue is and solve it by retrieving sperm from the epididymis or testicles via a thin needle. While it may be a little painful, it will make it possible for a man to get their woman pregnant through In Vitro Fertilization.